Protein Purification by Ion-Exchange Chromatography

List of Commercially Available Weak Cation Exchangers*

Last updated on January 1, 2013. REACH Devices does not manufacture, does not distribute, and does not promote any ion-exchange resins. We develop, manufacture and sell chromatography detectors and detector/gradient pump systems for FPLC, LPLC and MPLC.

Ion Exchanger type Functional group on IE matrix Charge Z of the functional groups
Abbreviation Name Structure pKa
pH of the solution
<2 2-2.5 2.5-3 3-5 5-7 7-10 10-11 >11
Weak cation exchanger CM Carboxymethyl -O-CH2-COO- 3.5-4.5 Z~0 -1<Z<0 Z=-1

*See main Ion-Exchange Chromatography page for detailed description.

  Diameter, μm:
mean (range)
Material Exclusion limit, (Pore size) Ionic
capacity, μeq/ml
Binding capacity, mg/ml
LysozymeE
(human IgG)ELysozyme: pI ~11, MW = 14.5kDa; Human IgG: pI~7.4-8.6, MW = 150kDa; Bovine COHb: MW = 69kDa.
Max
Pressure,
psi
pH stability:
long term
(short term)
Material
Trade name
1 400 (300-500) Agarose, 4% cross-linked, hardenedB2Supplier reports that volume changes due to ionic strength or pH are negligible. No data No data 50E1No data about the binding conditions. 9F1By analogy with data for Q-Cellthru Bigbeads Plus in A. Staby, I. H. Jensen, Journal of Chromatography A, 908, 2001, 149–161. 2-14 CM Cellthru BigBead Plus01Sterogene
2 128 (80-240) Dextran, cross-linkedB2Supplier reports that volume changes due to ionic strength or pH are negligible. 30kDa 480-600D1Supplier provides ionic capacity data as 4-5 mmol/g. Number in the table obtained by applying the experimentally established in column packing density of 0.12 dry g/ml ( P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J. Chromatography. A, 160, 1991, 207. ). (70 bovine-COHb)E2Flow rate of 75 cm/h in 0.1 M acetate buffer, pH 5.0. 2.5 2-12 CM Sephadex C-2502GE Life Sciences (former Amersham biosciences)
3 No data Cellulose, microgranular preswollenB1Supplier does not provide data about volume changes due to ionic strength or pH. No data 135-173D2Supplier provides ionic capacity data as 0.90-1.15 mmol/dry gram. Number in the table obtained by applying the experimentally established column packing density of 0.15 dry g/ml ( P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J. Chromatography. A, 160, 1991, 207. ). 177E3No flow rate reported, 10 mM acetate buffer, pH 5.0. Supplier provides binding capacity data as 1180 mg/dry gram. Number in the table obtained by applying the experimentally established column packing density of 0.15 dry g/ml ( P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J. Chromatography. A, 160, 1991, 207. ). No data 3-10 CM 5203Whatman (part of GE Healthcare)
4 60-130 fibers Cellulose, microgranularB2Supplier reports that volume changes due to ionic strength or pH are negligible. No data 240D3Supplier provides ionic capacity data as 1 meq/dry gram. Number in the table obtained by applying the experimentally established column packing density of 0.24 dry g/ml ( P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J. Chromatography. A, 160, 1991, 207. ). 162E1No data about the binding conditions. >10 4.5-10G1Working pH range. Express-Ion C03Whatman (part of GE Healthcare)
5 100 (50-150) Methacrylic polymerB2Supplier reports that volume changes due to ionic strength or pH are negligible. 5,000kDa (1,000Å) 90 25-45E1No data about the binding conditions. 43 2-13 Toyopearl CM-650C04TOSOH Biosep LLC (former TosoHaas)
6 90 [57AExperimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography , Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245.] (45–165) Agarose, 6% crosslinkedB2Supplier reports that volume changes due to ionic strength or pH are negligible. 4,000kDa (546Å)C1– Mean pore diameter ISEC-calculated in Ref.3 – P. DePhillips, A.M. Lenhoff, Journal of Chromatography A, 883, 2000, 39–54. 90–130 (30 bovine-COHb)E2Flow rate of 75 cm/h in 0.1 M acetate buffer, pH 5.0. 43 4-13 (2-14) CM Sepharose Fast Flow02GE Life Sciences (former Amersham biosciences)
7 73AExperimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography , Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245. (40 –130) Cellulose, cross-linked, beadedB2Supplier reports that volume changes due to ionic strength or pH are negligible. >500kDa 187D4Supplier provides ionic capacity data as 1.1 meq/dry gram. Number in the table obtained by applying the experimentally established column packing density of 0.17 dry g/ml ( P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J. Chromatography. A, 160, 1991, 207. ). >70E1No data about the binding conditions. 29 2-12 (1-14) Cellufine C-50005JNC corporation, Japan
8 (40 –130) Cellulose, cross-linked, dextran surface extenderB1Supplier does not provide data about volume changes due to ionic strength or pH. >1,000kDa 90-220 220E4Dynamic at 10 % Breakthrough, 43 2-13 Cellufine MAX CM05JNC corporation, Japan
9 75 (50-100) Methacrylic polymerB2Supplier reports that volume changes due to ionic strength or pH are negligible. 600kDa (500Å) 160-280 (100 human-IgG)E5Flow rate of 212 cm/h, 1mg/ml of polyclonal human Ig G in 50 mM sodium acetate buffer, pH 4.7. 43 3-13 Toyopearl GigaCap CM-65004TOSOH Biosep LLC (former TosoHaas)
10 67AExperimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography , Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245. (40-90) Methacrylic polymer, cross-linked, proprietary surface extenderB3Long, linear polymer chains (“tentacles") carry the functional ligands. (800Å) No data 80-120E1No data about the binding conditions. 116 1-13 Fractogel COO (M)06EMD Millipore (Merck Millipore)
11 65 [62AExperimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography , Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245.] (40-90) Methacrylic polymerB2Supplier reports that volume changes due to ionic strength or pH are negligible. 5,000kDa (1,000Å) 80-120 30-50E1No data about the binding conditions. 43 2-13 Toyopearl CM-650M04TOSOH Biosep LLC (former TosoHaas)
12 50 [44AExperimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography , Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245.] Ceramic scaffold filled w/ polymerized hydrogelB4Supplier reports the complete absence of volume changes due to ionic strength or pH. No data 250-400 (60 human-IgGkDa)E6No flow rate reported, 5 mg/mL Human IgG in 50 mM sodium acetate, pH 4.7. 1000 2-12 (1-14) CM Ceramic HyperD F07PALL Life Sciences (former BioSepra)
13 50 [62AExperimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography , Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245.] Methacrylic polymerB5Supplier reports <4% shrinkage/swelling on change from pure 0.1 to 1M NaCl. (1,000Å) 170-250 (>25 Hemo-globin)E1No data about the binding conditions. 102 1-12 Macro-Prep CM08Bio-Rad.
14 35 (20-40) Methacrylic polymerB2Supplier reports that volume changes due to ionic strength or pH are negligible. 5,000kDa (1,000Å) 80-120 30-50E1No data about the binding conditions. 43 2-13 Toyopearl CM-650S04TOSOH Biosep LLC (former TosoHaas)
15 34 (24-44) Agarose, highly cross-linked No data 60-80 No data >43 4-10G1 (2-14) CM Sepharose High Performance02GE Life Sciences (former Amersham biosciences)
16 (20-40) Methacrylic polymer, cross-linked, proprietary surface extenderB3Long, linear polymer chains (“tentacles") carry the functional ligands. (800Å) No data 120-180E1No data about the binding conditions. 116 1-13 Fractogel COO (S)06EMD Millipore (Merck Millipore)

A-Experimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography , Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245.

B1-Supplier does not provide data about volume changes due to ionic strength or pH. B2-Supplier reports that volume changes due to ionic strength or pH are negligible. B3-Long, linear polymer chains (“tentacles") carry the functional ligands. B4-Supplier reports the complete absence of volume changes due to ionic strength or pH. B5-Supplier reports <4% shrinkage/swelling on change from pure 0.1 to 1M NaCl.

C1– Mean pore diameter ISEC-calculated in Ref.3 – P. DePhillips, A.M. Lenhoff, Journal of Chromatography A, 883, 2000, 39–54.

D1-Supplier provides ionic capacity data as 4-5 mmol/g. Number in the table obtained by applying the experimentally established in column packing density of 0.12 dry g/ml (P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J. Chromatography. A, 160, 1991, 207.). D2-Supplier provides ionic capacity data as 0.90-1.15 mmol/dry gram. Number in the table obtained by applying the experimentally established column packing density of 0.15 dry g/ml (P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J. Chromatography. A, 160, 1991, 207.). D3-Supplier provides ionic capacity data as 1 meq/dry gram. Number in the table obtained by applying the experimentally established column packing density of 0.24 dry g/ml (P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J. Chromatography. A, 160, 1991, 207.). D4-Supplier provides ionic capacity data as 1.1 meq/dry gram. Number in the table obtained by applying the experimentally established column packing density of 0.17 dry g/ml (P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J. Chromatography. A, 160, 1991, 207.).

E-Lysozyme: pI ~11, MW = 14.5kDa; Human IgG: pI~7.4-8.6, MW = 150kDa; Bovine COHb: MW = 69kDa. E1-No data about the binding conditions. E2-Flow rate of 75 cm/h in 0.1 M acetate buffer, pH 5.0. E3-No flow rate reported, 10 mM acetate buffer, pH 5.0. Supplier provides binding capacity data as 1180 mg/dry gram. Number in the table obtained by applying the experimentally established column packing density of 0.15 dry g/ml (P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J. Chromatography. A, 160, 1991, 207.). E4-Dynamic at 10 % Breakthrough, E5-Flow rate of 212 cm/h, 1mg/ml of polyclonal human Ig G in 50 mM sodium acetate buffer, pH 4.7. E6-No flow rate reported, 5 mg/mL Human IgG in 50 mM sodium acetate, pH 4.7.

F1-By analogy with data for Q-Cellthru Bigbeads Plus in A. Staby, I. H. Jensen, Journal of Chromatography A, 908, 2001, 149–161.

G1-Working pH range.

01-Sterogene 02-GE Life Sciences (former Amersham biosciences) 03-Whatman (part of GE Healthcare) 04-TOSOH Biosep LLC (former TosoHaas) 05-JNC corporation, Japan 06-EMD Millipore (Merck Millipore) 07-PALL Life Sciences (former BioSepra) 08-Bio-Rad.