Protein Purification by Ion-Exchange Chromatography

List of Commercially Available Strong Cation Exchangers*

Last updated on January 1, 2013. REACH Devices does not manufacture, does not distribute, and does not promote any ion-exchange resins. We develop, manufacture and sell chromatography detectors and detector/gradient pump systems for FPLC, LPLC and MPLC.

Ion Exchanger type Functional group on IE matrix Charge Z of the functional groups
Abbreviation Name Structure pKa
pH of the solution
<2 2-2.5 2.5-3 3-5 5-7 7-10 10-11 >11
Strong cation exchanger SP Sulfopropyl -O-CH2-CH2-CH2-SO3- 2-2.5 Z~0 Z=-1
SE Sulfoethyl -O-CH2-CH2-SO3- 2 Z~0 Z=-1
S Sulfomethyl -O-CH2-SO3- 2

*See main Ion-Exchange Chromatography page for detailed description.

  Diameter, μm:
mean (range)
Material Exclusion limit, (Pore size) Ionic
capacity, μeq/ml
Binding capacity, mg/ml
LysozymeE
(human IgG)ELysozyme: pI ~11, MW = 14.5 kDa; Human IgG: pI~7.4-8.6, MW = 150 kDa; Bovine COHb: MW = 69 kDa; Ribonuclease: pI~9.6, MW = 14 kDA; Insulin: pI~5.3, MW = 6kDa.
Max
Pressure,
psi
pH stability:
long term
(short term)
Material
Trade name
1 400 (300-500) Agarose, 4% cross-linked, hardenedB2Supplier reports that volume changes due to ionic strength or pH are negligible. No data No data 50E1No data about the binding conditions. 9F1By analogy with data for Q-Cellthru Bigbeads Plus: A. Staby, I. H. Jensen, Journal of Chromatography A, 908, 2001, 149-161. 2-14 SP Cellthru BigBead Plus01Sterogene
2 200 (100-300) Agarose, 6% cross-inked, quartz coreB1Supplier does not provide data about volume changes due to ionic strength or pH. 4,000kDa 170-240 >60E1No data about the binding conditions. No data 4-13 (3-14) Streamline SP02GE Life Sciences (former Amersham biosciences)
3 200 (100-300) Agarose, 6% cross-linked, quartz core, dextran surface extenderB1Supplier does not provide data about volume changes due to ionic strength or pH. 4,000kDa 180-240 >140E1No data about the binding conditions. No data 4-13 (3-14) Streamline SP XL02GE Life Sciences (former Amersham biosciences)
3 200 [99AExperimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245. ] (100-300) Agarose, 6% cross-linked 4,000kDa 180-250 >92E2Dynamic at 10 % breakthrough: A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193-245. 43 4-13 (3-14) SP Sepharose Big Beads02GE Life Sciences (former Amersham biosciences)
4 200 (100-300) Methacrylic polymerB2Supplier reports that volume changes due to ionic strength or pH are negligible. 100kDa (500Å) 140-180 70E3Dynamic at 10 % breakthrough. 43F2Value is provided in the Tosoh website. Material data sheet (downloaded from the same website) give max pressure value of 102 psi. 2-13 Toyopearl M-Cap II SP-550EC04TOSOH Biosep LLC (former TosoHaas)
5 125 (40-125 dry) Dextran, cross-linkedB2Supplier reports that volume changes due to ionic strength or pH are negligible. 30kDa 390-510D1Supplier provides ionic capacity data as 2.6-3.4 meq/dry gram. Number in the table obtained by applying the experimentally established column packing density of 0.15 dry g/ml: P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J.Chromatography. A,160, 1991, 207. 70E4(bovine COHbELysozyme: pI ~11, MW = 14.5 kDa; Human IgG: pI~7.4-8.6, MW = 150 kDa; Bovine COHb: MW = 69 kDa; Ribonuclease: pI~9.6, MW = 14 kDA; Insulin: pI~5.3, MW = 6kDa.) 2.5 2-12 SP Sephadex A-2502GE Life Sciences (former Amersham biosciences)
6 60-130 fibers Cellulose, microgranularB2Supplier reports that volume changes due to ionic strength or pH are negligible. sulfoxyethyl No data 220D2Supplier provides ionic capacity data as 1 meq/dry gram. Number in the table obtained by applying the experimentally established column packing density of 0.22 dry g/ml: P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J.Chromatography. A,160, 1991, 207. 153E1No data about the binding conditions. >10 2-12G1Working pH range. Express-Ion S03Whatman (part of GE Healthcare)
7 100 [80AExperimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245. ] (50-150) Methacrylic polymerB2Supplier reports that volume changes due to ionic strength or pH are negligible. 100kDa (500Å) 140-180 70E3Dynamic at 10 % breakthrough. 43F2Value is provided in the Tosoh website. Material data sheet (downloaded from the same website) give max pressure value of 102 psi. 2-13 Toyopearl SP-550C04TOSOH Biosep LLC (former TosoHaas)
8 100 [80AExperimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245. ] (50-150) Methacrylic polymerB2Supplier reports that volume changes due to ionic strength or pH are negligible. 5,000kDa (1,000Å) 120-180 35E3Dynamic at 10 % breakthrough. 43F2Value is provided in the Tosoh website. Material data sheet (downloaded from the same website) give max pressure value of 102 psi. 2-13 Toyopearl SP-650C04TOSOH Biosep LLC (former TosoHaas)
9 90 [66AExperimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245. ] (45-165) Agarose, 6% crosslinkedB2Supplier reports that volume changes due to ionic strength or pH are negligible. 4,000kDa (500Å)C1Mean pore diameter ISEC-calculated in: P. DePhillips, A.M. Lenhoff , Journal of Chromatography A, 883, 2000,39-54. 180-250 104 (50)E5Lysozyme: Dynamic at 10 % Breakthrough: A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193-245. Human IgG: Flow rate of 75 cm/h in 0.1 M acetate bugger pH 5. 43 4-13 (3-14) SP Sepharose Fast Flow02GE Life Sciences (former Amersham biosciences)
10 90 [66AExperimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245. ] (45-165) Agarose, 6% cross-linked, dextran surface extenderB2Supplier reports that volume changes due to ionic strength or pH are negligible. (120Å)C2By analogy with mean pore diameter for Q Sepharose XL stated in: Y. Yao, A.M. Lenhoff , Journal of Chromatography A, 1037, 2004,273-282. 180-250 >160E1No data about the binding conditions. 43 4-13 (3-14) SP Sepharose XL02GE Life Sciences (former Amersham biosciences)
11 90 Agarose, cross-linked, dextran surface extenderB1Supplier does not provide data about volume changes due to ionic strength or pH. No dataC3Supplier states that the bead and pore sizes are similar between Capto S and Q Sepharose™ Fast Flow. 110-140 >120E6Dynamic at 10 % Breakthrough. Flow rate of 600 cm/h in 30 mM sodium phosphate buffer, pH 6.8. 43 4-13 (3-14) Capto S02GE Life Sciences (former Amersham biosciences)
12 90 (40-130) Cellulose, cross-linked, dextran surface extender,B1Supplier does not provide data about volume changes due to ionic strength or pH. sulfoxybuthyl >1,000kDa 120-240 144(131)E2Dynamic at 10 % breakthrough: A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193-245. 43 2-13 Cellufine MAX S-r05JNC corporation, Japan
13 90 (40-130) Cellulose, cross-linked, dextran surface extender,B1Supplier does not provide data about volume changes due to ionic strength or pH. sulfoxybuthyl >1,000kDa 130-250 191 (216)E3Dynamic at 10 % breakthrough. 43 3-14 Cellufine MAX S-h05JNC corporation, Japan
14 85 (70-100) Acrylamide/vinyl copolymer, proprietary surface extender B1Supplier does not provide data about volume changes due to ionic strength or pH. 40kDa (100Å)C4Determined by inverse size exclusion chromatography: E. X. Pérez Almodóvar, Y. Tao, G. Carta, Biotechnol Prog. 27, 2011, 1264-72. 90-150 320 (110)E7Lysozyme: batch adsorption, sodium phosphate buffer pH 6.5: E. X. Pérez Almodóvar, Y. Tao, G. Carta, Biotechnol Prog. 27, 2011, 1264–72. Human IgG: dynamic at 10 % breakthrough, flow rate of 300 cm/h, 4.5 mg/ml polyclonal human IgG in 40 mM sodium acetate and 30 mM sodium chloride, pH 5.0. 29 2-14 (4-13) Nuvia S08Bio-Rad
15 (75-95) Vinyl ether polymer, proprietary surface extender B1Supplier does not provide data about volume changes due to ionic strength or pH. No data 50-100 115-165 (>60)E8Lysozyme: no data about the binding conditions. Human IgG: dynamic, 2 min residence time. No data No data Eshmuno S Resin06EMD Millipore (Merck Millipore)
16 80 Acrylamide/vinyl copolymerB3Supplier reports <5% volume changes at pH 4-10 and for 0.01-1.0 M NaCl. (1,400Å)C4Determined by inverse size exclusion chromatography: E. X. Pérez Almodóvar, Y. Tao, G. Carta, Biotechnol Prog. 27, 2011, 1264-72. 250-310 160 (60)E9Lysozyme: batch adsorption, sodium phosphate buffer pH 6.5: E. X. Pérez Almodóvar, Y. Tao, G. Carta, Biotechnol Prog. 27, 2011, 1264–72. Human IgG: 10 % breakthrough, flow rate of 150 cm/h, 4.5 mg/ml polyclonal human IgG in 40 mM sodium acetate and 30 mM sodium chloride, pH 5.0. 29 1-14 UNOsphere S08Bio-Rad
17 80 Acrylamide/vinyl copolymerB3Supplier reports <5% volume changes at pH 4-10 and for 0.01-1.0 M NaCl. No data 140 (60)E10Human IgG: dynamic at 10 % breakthrough, flow rate of 150 cm/h, 4.5 mg/ml polyclonal human IgG in 40 mM sodium acetate and 30 mM sodium chloride, pH 5.0. 29 2-13 (1-14) UNOsphere Rapid S08Bio-Rad
18 75 (50-100) Methacrylic polymerB2Supplier reports that volume changes due to ionic strength or pH are negligible. (1,000Å) 160 200 (145)E11Lysozyme: static. Human IgG: dynamic at 10 % breakthrough, flow rate of 212 cm/h, 1 mg/ml polyclonal human IgG in 100 mM acetate buffer. 43 3-13 Toyopearl Giga-Cap S-650 (M)04TOSOH Biosep LLC (former TosoHaas)
19 75? (60-90) Cellulose matrixB1Supplier does not provide data about volume changes due to ionic strength or pH. No data 59-84 (134-190)E122 Minute residence time, 5 mg/mL hIgG in 50 mM sodium acetate, pH 4.5. 19 1-13 (1-14) S HyperCel Sorbent 07PALL Life Sciences (former BioSepra)
20 65 [62AExperimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245. ] (40-90) Methacrylic polymerB2Supplier reports that volume changes due to ionic strength or pH are negligible. 5,000kDa (1,000Å) 130-170 35E3Dynamic at 10 % breakthrough. 43F2Value is provided in the Tosoh website. Material data sheet (downloaded from the same website) give max pressure value of 102 psi. 2-13 Toyopearl SP-650M04TOSOH Biosep LLC (former TosoHaas)
21 50 [61AExperimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245. ] Methacrylic polymerB4Supplier reports <3% volume changes at pH 4-10 and <9% volume changes for 0.01-1.0 M NaCl. (1,000Å) 120-200 40 (>49)E13Lysozyme: Dynamic at 10 % Breakthrough, A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193-245. Human IgG: no data about the binding conditions. 102 1-10 Macro-Prep High S08Bio-Rad
22 [59AExperimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245. ] (40-90) Methacrylic polymer, cross-linked, proprietary surface extenderB1Supplier does not provide data about volume changes due to ionic strength or pH. (800Å) No data 120-160E1No data about the binding conditions. 116 2-12 (1-13) Fractogel SE Hicap (M)06EMD Millipore (Merck Millipore)
23 [57AExperimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245. ] (40-90) Methacrylic polymer, cross-linked, proprietary surface extenderB1Supplier does not provide data about volume changes due to ionic strength or pH. (800Å) No data 130E1No data about the binding conditions. 116 2-12 (1-13) Fractogel SO3(M)06EMD Millipore (Merck Millipore)
24 50 [57AExperimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245. ] Ceramic scaffold filled w/ polymerized hydrogelB5Supplier reports that volume changes due to ionic strength or pH are absent. No data >150 >75E14Dynamic at 10 % breakthrough, flow rate of 200 cm/h, 5 mg/mL lysozyme in 50 mM sodium acetate, pH 4.5. 1,000 2-12 (1-14) S Ceramic HyperD F07PALL Life Sciences (former BioSepra)
25 50 Acrylamide-dextran copolymerB1Supplier does not provide data about volume changes due to ionic strength or pH. No data 100-130 No dataE15The supplier recommends the media for purifications of proteins in excess of 150kDa, and for purifications of PEGylated proteins containing at least 10kDa of PEG. 43 4-11 (2-13) MacroCap SP02GE Life Sciences (former Amersham biosciences)
26 40 (36-44) Agarose, high-flowB1Supplier does not provide data about volume changes due to ionic strength or pH. No data 130-160 >70E16Dynamic at 10 % breakthrough, residence time of 4 min at flow rate of 250 cm/h, 20 mM sodium phosphate, pH 7.2. 43 4-12 (3-14) Capto SP ImpRes02GE Life Sciences (former Amersham biosciences)
27 35 (20-50) Methacrylic polymerB2Supplier reports that volume changes due to ionic strength or pH are negligible. 5,000kDa (1,000Å) 130-170 35E2Dynamic at 10 % breakthrough: A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193-245. 43F2Value is provided in the Tosoh website. Material data sheet (downloaded from the same website) give max pressure value of 102 psi. 2-13 Toyopearl SP-650S04TOSOH Biosep LLC (former TosoHaas)
28 34 (24-44) Agarose, 6% crosslinkedB2Supplier reports that volume changes due to ionic strength or pH are negligible. 4,000kDa 140-200 55 (RibonucleaseELysozyme: pI ~11, MW = 14.5 kDa; Human IgG: pI~7.4-8.6, MW = 150 kDa; Bovine COHb: MW = 69 kDa; Ribonuclease: pI~9.6, MW = 14 kDA; Insulin: pI~5.3, MW = 6kDa.) 72 4-13 (3-14) SP Sepharose High Perform.02GE Life Sciences (former Amersham biosciences)
29 30 Methacrylic polymerB2Supplier reports that volume changes due to ionic strength or pH are negligible. 250kDa (250Å) 70-220 >65 (InsulinE)E1No data about the binding conditions. 290 2-13 TSKgel SP-3PW04TOSOH Biosep LLC (former TosoHaas)
30 30 (20-40) Methacrylic polymerB2Supplier reports that volume changes due to ionic strength or pH are negligible. 5,000kDa (1,000Å) 60-120 20-40E1No data about the binding conditions. 290 2-13 TSKgel SP-5PW (30µm)04TOSOH Biosep LLC (former TosoHaas)
31 (20-40) Methacrylic polymer, cross-linked, proprietary surface extenderB1Supplier does not provide data about volume changes due to ionic strength or pH. (800Å) No data 150E1No data about the binding conditions. 116 1-13 Fractogel SO3(S)06EMD Millipore (Merck Millipore)
32 25 [20AExperimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245. ] Methacrylic polymerB6Supplier reports <1% volume changes at pH 4-10 and <5% volume changes for 0.01-1.0 M NaCl. (725Å) 90-150 (>40)E17Dynamic. 100 1-12 Macro-Prep 25S08Bio-Rad

A-Experimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245.

B1-Supplier does not provide data about volume changes due to ionic strength or pH. B2-Supplier reports that volume changes due to ionic strength or pH are negligible. B3-Supplier reports <5% volume changes at pH 4-10 and for 0.01-1.0 M NaCl. B4-Supplier reports <3% volume changes at pH 4-10 and <9% volume changes for 0.01-1.0 M NaCl. B5-Supplier reports that volume changes due to ionic strength or pH are absent. B6-Supplier reports <1% volume changes at pH 4-10 and <5% volume changes for 0.01-1.0 M NaCl.

C1-Mean pore diameter ISEC-calculated in:P. DePhillips, A.M. Lenhoff , Journal of Chromatography A, 883, 2000,39-54. C2-By analogy with mean pore diameter for Q Sepharose XL stated in: Y. Yao, A.M. Lenhoff , Journal of Chromatography A, 1037, 2004,273-282. C3-Supplier states that the bead and pore sizes are similar between Capto S and Q Sepharose™ Fast Flow. C4-Determined by inverse size exclusion chromatography: E. X. Pérez Almodóvar, Y. Tao, G. Carta, Biotechnol Prog. 27, 2011, 1264-72.

D1-Supplier provides ionic capacity data as 2.6-3.4 meq/dry gram. Number in the table obtained by applying the experimentally established column packing density of 0.15 dry g/ml: P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J.Chromatography. A,160, 1991, 207. D2-Supplier provides ionic capacity data as 1 meq/dry gram. Number in the table obtained by applying the experimentally established column packing density of 0.22 dry g/ml: P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J.Chromatography. A,160, 1991, 207.

E-Lysozyme: pI ~11, MW = 14.5 kDa; Human IgG: pI~7.4-8.6, MW = 150 kDa; Bovine COHb: MW = 69 kDa; Ribonuclease: pI~9.6, MW = 14 kDA; Insulin: pI~5.3, MW = 6kDa. E1-No data about the binding conditions. E2-Dynamic at 10 % breakthrough: A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193-245. E3-Dynamic at 10 % breakthrough. E4-Flow rate of 75 cm/h in 0.1 M acetate bugger pH 5. Only 1.1 mg/ml of IgG binding catacity at the same conditions. E5-Lysozyme: Dynamic at 10 % Breakthrough: A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193-245. Human IgG: Flow rate of 75 cm/h in 0.1 M acetate bugger pH 5. E6-Dynamic at 10 % Breakthrough. Flow rate of 600 cm/h in 30 mM sodium phosphate buffer, pH 6.8. E7-Lysozyme: batch adsorption, sodium phosphate buffer pH 6.5:E. X. Pérez Almodóvar, Y. Tao, G. Carta, Biotechnol Prog. 27, 2011, 1264–72. Human IgG: dynamic at 10 % breakthrough, flow rate of 300 cm/h, 4.5 mg/ml polyclonal human IgG in 40 mM sodium acetate and 30 mM sodium chloride, pH 5.0. E8-Lysozyme: no data about the binding conditions. Human IgG: dynamic, 2 min residence time. E9-Lysozyme: batch adsorption, sodium phosphate buffer pH 6.5:E. X. Pérez Almodóvar, Y. Tao, G. Carta, Biotechnol Prog. 27, 2011, 1264–72. Human IgG: 10 % breakthrough, flow rate of 150 cm/h, 4.5 mg/ml polyclonal human IgG in 40 mM sodium acetate and 30 mM sodium chloride, pH 5.0. E10-Human IgG: dynamic at 10 % breakthrough, flow rate of 150 cm/h, 4.5 mg/ml polyclonal human IgG in 40 mM sodium acetate and 30 mM sodium chloride, pH 5.0. E11-Lysozyme: static. Human IgG: dynamic at 10 % breakthrough, flow rate of 212 cm/h, 1 mg/ml polyclonal human IgG in 100 mM acetate buffer. E12-2 Minute residence time, 5 mg/mL hIgG in 50 mM sodium acetate, pH 4.5. E13-Lysozyme: Dynamic at 10 % Breakthrough, A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193-245. Human IgG: no data about the binding conditions. E14-Dynamic at 10 % breakthrough, flow rate of 200 cm/h, 5 mg/mL lysozyme in 50 mM sodium acetate, pH 4.5. E15-The supplier recommends the media for purifications of proteins in excess of 150kDa, and for purifications of PEGylated proteins containing at least 10kDa of PEG. E16-Dynamic at 10 % breakthrough, residence time of 4 min at flow rate of 250 cm/h, 20 mM sodium phosphate, pH 7.2. E17-Dynamic.

F1-By analogy with data for Q-Cellthru Bigbeads Plus: A. Staby, I. H. Jensen, Journal of Chromatography A, 908, 2001, 149-161. F2-Value is provided in the Tosoh website. Material data sheet (downloaded from the same website) give max pressure value of 102 psi.

G1-Working pH range.

01-Sterogene 02-GE Life Sciences (former Amersham biosciences) 03-Whatman (part of GE Healthcare) 04-TOSOH Biosep LLC (former TosoHaas) 05-JNC corporation, Japan 06-EMD Millipore (Merck Millipore) 07-PALL Life Sciences (former BioSepra) 08-Bio-Rad