Protein Purification by Ion-Exchange Chromatography

List of Commercially Available Strong Cation Exchangers^*

Last updated on January 1, 2013. REACH Devices does not manufacture, does not distribute, and does not promote any ion-exchange resins. We develop, manufacture and sell chromatography detectors and detector/gradient pump systems for FPLC, LPLC and MPLC.

2-2.5

2.5-3

3-5

5-7

7-10

10-11

>11

Strong cation exchanger

Sulfopropyl

-O-CH₂-CH₂-CH₂-SO₃^-

2-2.5

Z~0

Z=-1

Sulfoethyl

-O-CH₂-CH₂-SO₃^-

Z~0

Z=-1

Sulfomethyl

-O-CH₂-SO₃^-

^*See main Ion-Exchange Chromatography page for detailed description.

	Diameter, μm: mean (range)	Material	Exclusion limit, (Pore size)	Ionic capacity, μeq/ml	Binding capacity, mg/ml Lysozyme^E (human IgG)^{ELysozyme: pI ~11, MW = 14.5 kDa; Human IgG: pI~7.4-8.6, MW = 150 kDa; Bovine COHb: MW = 69 kDa; Ribonuclease: pI~9.6, MW = 14 kDA; Insulin: pI~5.3, MW = 6kDa.}	Max Pressure, psi	pH stability: long term (short term)	Material Trade name
1	400 (300-500)	Agarose, 4% cross-linked, hardened^{B2Supplier reports that volume changes due to ionic strength or pH are negligible.}	No data	No data	50^{E1No data about the binding conditions.}	9^{F1By analogy with data for Q-Cellthru Bigbeads Plus: A. Staby, I. H. Jensen, Journal of Chromatography A, 908, 2001, 149-161.}	2-14	SP Cellthru BigBead Plus^01Sterogene
2	200 (100-300)	Agarose, 6% cross-inked, quartz core^{B1Supplier does not provide data about volume changes due to ionic strength or pH.}	4,000kDa	170-240	>60^{E1No data about the binding conditions.}	No data	4-13 (3-14)	Streamline SP^{02GE Life Sciences (former Amersham biosciences)}
3	200 (100-300)	Agarose, 6% cross-linked, quartz core, dextran surface extender^{B1Supplier does not provide data about volume changes due to ionic strength or pH.}	4,000kDa	180-240	>140^{E1No data about the binding conditions.}	No data	4-13 (3-14)	Streamline SP XL^{02GE Life Sciences (former Amersham biosciences)}
3	200 [99^{AExperimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245.}] (100-300)	Agarose, 6% cross-linked	4,000kDa	180-250	>92^{E2Dynamic at 10 % breakthrough: A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193-245.}	43	4-13 (3-14)	SP Sepharose Big Beads^{02GE Life Sciences (former Amersham biosciences)}
4	200 (100-300)	Methacrylic polymer^{B2Supplier reports that volume changes due to ionic strength or pH are negligible.}	100kDa (500Å)	140-180	70^{E3Dynamic at 10 % breakthrough.}	43^{F2Value is provided in the Tosoh website. Material data sheet (downloaded from the same website) give max pressure value of 102 psi.}	2-13	Toyopearl M-Cap II SP-550EC^{04TOSOH Biosep LLC (former TosoHaas)}
5	125 (40-125 dry)	Dextran, cross-linked^{B2Supplier reports that volume changes due to ionic strength or pH are negligible.}	30kDa	390-510^{D1Supplier provides ionic capacity data as 2.6-3.4 meq/dry gram. Number in the table obtained by applying the experimentally established column packing density of 0.15 dry g/ml: P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J.Chromatography. A,160, 1991, 207.}	70^E4(bovine COHb^{ELysozyme: pI ~11, MW = 14.5 kDa; Human IgG: pI~7.4-8.6, MW = 150 kDa; Bovine COHb: MW = 69 kDa; Ribonuclease: pI~9.6, MW = 14 kDA; Insulin: pI~5.3, MW = 6kDa.})	2.5	2-12	SP Sephadex A-25^{02GE Life Sciences (former Amersham biosciences)}
6	60-130 fibers	Cellulose, microgranular^{B2Supplier reports that volume changes due to ionic strength or pH are negligible.} sulfoxyethyl	No data	220^{D2Supplier provides ionic capacity data as 1 meq/dry gram. Number in the table obtained by applying the experimentally established column packing density of 0.22 dry g/ml: P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J.Chromatography. A,160, 1991, 207.}	153^{E1No data about the binding conditions.}	>10	2-12^{G1Working pH range.}	Express-Ion S^{03Whatman (part of GE Healthcare)}
7	100 [80^{AExperimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245.}] (50-150)	Methacrylic polymer^{B2Supplier reports that volume changes due to ionic strength or pH are negligible.}	100kDa (500Å)	140-180	70^{E3Dynamic at 10 % breakthrough.}	43^{F2Value is provided in the Tosoh website. Material data sheet (downloaded from the same website) give max pressure value of 102 psi.}	2-13	Toyopearl SP-550C^{04TOSOH Biosep LLC (former TosoHaas)}
8	100 [80^{AExperimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245.}] (50-150)	Methacrylic polymer^{B2Supplier reports that volume changes due to ionic strength or pH are negligible.}	5,000kDa (1,000Å)	120-180	35^{E3Dynamic at 10 % breakthrough.}	43^{F2Value is provided in the Tosoh website. Material data sheet (downloaded from the same website) give max pressure value of 102 psi.}	2-13	Toyopearl SP-650C^{04TOSOH Biosep LLC (former TosoHaas)}
9	90 [66^{AExperimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245.}] (45-165)	Agarose, 6% crosslinked^{B2Supplier reports that volume changes due to ionic strength or pH are negligible.}	4,000kDa (500Å)^{C1Mean pore diameter ISEC-calculated in: P. DePhillips, A.M. Lenhoff , Journal of Chromatography A, 883, 2000,39-54.}	180-250	104 (50)^{E5Lysozyme: Dynamic at 10 % Breakthrough: A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193-245. Human IgG: Flow rate of 75 cm/h in 0.1 M acetate bugger pH 5.}	43	4-13 (3-14)	SP Sepharose Fast Flow^{02GE Life Sciences (former Amersham biosciences)}
10	90 [66^{AExperimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245.}] (45-165)	Agarose, 6% cross-linked, dextran surface extender^{B2Supplier reports that volume changes due to ionic strength or pH are negligible.}	(120Å)^{C2By analogy with mean pore diameter for Q Sepharose XL stated in: Y. Yao, A.M. Lenhoff , Journal of Chromatography A, 1037, 2004,273-282.}	180-250	>160^{E1No data about the binding conditions.}	43	4-13 (3-14)	SP Sepharose XL^{02GE Life Sciences (former Amersham biosciences)}
11	90	Agarose, cross-linked, dextran surface extender^{B1Supplier does not provide data about volume changes due to ionic strength or pH.}	No data^{C3Supplier states that the bead and pore sizes are similar between Capto S and Q Sepharose™ Fast Flow.}	110-140	>120^{E6Dynamic at 10 % Breakthrough. Flow rate of 600 cm/h in 30 mM sodium phosphate buffer, pH 6.8.}	43	4-13 (3-14)	Capto S^{02GE Life Sciences (former Amersham biosciences)}
12	90 (40-130)	Cellulose, cross-linked, dextran surface extender,^{B1Supplier does not provide data about volume changes due to ionic strength or pH.} sulfoxybuthyl	>1,000kDa	120-240	144(131)^{E2Dynamic at 10 % breakthrough: A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193-245.}	43	2-13	Cellufine MAX S-r^{05JNC corporation, Japan}
13	90 (40-130)	Cellulose, cross-linked, dextran surface extender,^{B1Supplier does not provide data about volume changes due to ionic strength or pH.}sulfoxybuthyl	>1,000kDa	130-250	191 (216)^{E3Dynamic at 10 % breakthrough.}	43	3-14	Cellufine MAX S-h^{05JNC corporation, Japan}
14	85 (70-100)	Acrylamide/vinyl copolymer, proprietary surface extender ^{B1Supplier does not provide data about volume changes due to ionic strength or pH.}	40kDa (100Å)^{C4Determined by inverse size exclusion chromatography: E. X. Pérez Almodóvar, Y. Tao, G. Carta, Biotechnol Prog. 27, 2011, 1264-72.}	90-150	320 (110)^{E7Lysozyme: batch adsorption, sodium phosphate buffer pH 6.5: E. X. Pérez Almodóvar, Y. Tao, G. Carta, Biotechnol Prog. 27, 2011, 1264–72. Human IgG: dynamic at 10 % breakthrough, flow rate of 300 cm/h, 4.5 mg/ml polyclonal human IgG in 40 mM sodium acetate and 30 mM sodium chloride, pH 5.0.}	29	2-14 (4-13)	Nuvia S^08Bio-Rad
15	(75-95)	Vinyl ether polymer, proprietary surface extender ^{B1Supplier does not provide data about volume changes due to ionic strength or pH.}	No data	50-100	115-165 (>60)^{E8Lysozyme: no data about the binding conditions. Human IgG: dynamic, 2 min residence time.}	No data	No data	Eshmuno S Resin^{06EMD Millipore (Merck Millipore)}
16	80	Acrylamide/vinyl copolymer^{B3Supplier reports <5% volume changes at pH 4-10 and for 0.01-1.0 M NaCl.}	(1,400Å)^{C4Determined by inverse size exclusion chromatography: E. X. Pérez Almodóvar, Y. Tao, G. Carta, Biotechnol Prog. 27, 2011, 1264-72.}	250-310	160 (60)^{E9Lysozyme: batch adsorption, sodium phosphate buffer pH 6.5: E. X. Pérez Almodóvar, Y. Tao, G. Carta, Biotechnol Prog. 27, 2011, 1264–72. Human IgG: 10 % breakthrough, flow rate of 150 cm/h, 4.5 mg/ml polyclonal human IgG in 40 mM sodium acetate and 30 mM sodium chloride, pH 5.0.}	29	1-14	UNOsphere S^08Bio-Rad
17	80	Acrylamide/vinyl copolymer^{B3Supplier reports <5% volume changes at pH 4-10 and for 0.01-1.0 M NaCl.}	No data	140	(60)^{E10Human IgG: dynamic at 10 % breakthrough, flow rate of 150 cm/h, 4.5 mg/ml polyclonal human IgG in 40 mM sodium acetate and 30 mM sodium chloride, pH 5.0.}	29	2-13 (1-14)	UNOsphere Rapid S^08Bio-Rad
18	75 (50-100)	Methacrylic polymer^{B2Supplier reports that volume changes due to ionic strength or pH are negligible.}	(1,000Å)	160	200 (145)^{E11Lysozyme: static. Human IgG: dynamic at 10 % breakthrough, flow rate of 212 cm/h, 1 mg/ml polyclonal human IgG in 100 mM acetate buffer.}	43	3-13	Toyopearl Giga-Cap S-650 (M)^{04TOSOH Biosep LLC (former TosoHaas)}
19	75? (60-90)	Cellulose matrix^{B1Supplier does not provide data about volume changes due to ionic strength or pH.}	No data	59-84	(134-190)^{E122 Minute residence time, 5 mg/mL hIgG in 50 mM sodium acetate, pH 4.5.}	19	1-13 (1-14)	S HyperCel Sorbent ^{07PALL Life Sciences (former BioSepra)}
20	65 [62^{AExperimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245.}] (40-90)	Methacrylic polymer^{B2Supplier reports that volume changes due to ionic strength or pH are negligible.}	5,000kDa (1,000Å)	130-170	35^{E3Dynamic at 10 % breakthrough.}	43^{F2Value is provided in the Tosoh website. Material data sheet (downloaded from the same website) give max pressure value of 102 psi.}	2-13	Toyopearl SP-650M^{04TOSOH Biosep LLC (former TosoHaas)}
21	50 [61^{AExperimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245.}]	Methacrylic polymer^{B4Supplier reports <3% volume changes at pH 4-10 and <9% volume changes for 0.01-1.0 M NaCl.}	(1,000Å)	120-200	40 (>49)^{E13Lysozyme: Dynamic at 10 % Breakthrough, A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193-245. Human IgG: no data about the binding conditions.}	102	1-10	Macro-Prep High S^08Bio-Rad
22	[59^{AExperimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245.}] (40-90)	Methacrylic polymer, cross-linked, proprietary surface extender^{B1Supplier does not provide data about volume changes due to ionic strength or pH.}	(800Å)	No data	120-160^{E1No data about the binding conditions.}	116	2-12 (1-13)	Fractogel SE Hicap (M)^{06EMD Millipore (Merck Millipore)}
23	[57^{AExperimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245.}] (40-90)	Methacrylic polymer, cross-linked, proprietary surface extender^{B1Supplier does not provide data about volume changes due to ionic strength or pH.}	(800Å)	No data	130^{E1No data about the binding conditions.}	116	2-12 (1-13)	Fractogel SO₃(M)^{06EMD Millipore (Merck Millipore)}
24	50 [57^{AExperimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245.}]	Ceramic scaffold filled w/ polymerized hydrogel^{B5Supplier reports that volume changes due to ionic strength or pH are absent.}	No data	>150	>75^{E14Dynamic at 10 % breakthrough, flow rate of 200 cm/h, 5 mg/mL lysozyme in 50 mM sodium acetate, pH 4.5.}	1,000	2-12 (1-14)	S Ceramic HyperD F^{07PALL Life Sciences (former BioSepra)}
25	50	Acrylamide-dextran copolymer^{B1Supplier does not provide data about volume changes due to ionic strength or pH.}	No data	100-130	No data^{E15The supplier recommends the media for purifications of proteins in excess of 150kDa, and for purifications of PEGylated proteins containing at least 10kDa of PEG.}	43	4-11 (2-13)	MacroCap SP^{02GE Life Sciences (former Amersham biosciences)}
26	40 (36-44)	Agarose, high-flow^{B1Supplier does not provide data about volume changes due to ionic strength or pH.}	No data	130-160	>70^{E16Dynamic at 10 % breakthrough, residence time of 4 min at flow rate of 250 cm/h, 20 mM sodium phosphate, pH 7.2.}	43	4-12 (3-14)	Capto SP ImpRes^{02GE Life Sciences (former Amersham biosciences)}
27	35 (20-50)	Methacrylic polymer^{B2Supplier reports that volume changes due to ionic strength or pH are negligible.}	5,000kDa (1,000Å)	130-170	35^{E2Dynamic at 10 % breakthrough: A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193-245.}	43^{F2Value is provided in the Tosoh website. Material data sheet (downloaded from the same website) give max pressure value of 102 psi.}	2-13	Toyopearl SP-650S^{04TOSOH Biosep LLC (former TosoHaas)}
28	34 (24-44)	Agarose, 6% crosslinked^{B2Supplier reports that volume changes due to ionic strength or pH are negligible.}	4,000kDa	140-200	55 (Ribonuclease^{ELysozyme: pI ~11, MW = 14.5 kDa; Human IgG: pI~7.4-8.6, MW = 150 kDa; Bovine COHb: MW = 69 kDa; Ribonuclease: pI~9.6, MW = 14 kDA; Insulin: pI~5.3, MW = 6kDa.})	72	4-13 (3-14)	SP Sepharose High Perform.^{02GE Life Sciences (former Amersham biosciences)}
29	30	Methacrylic polymer^{B2Supplier reports that volume changes due to ionic strength or pH are negligible.}	250kDa (250Å)	70-220	>65 (Insulin^E)^{E1No data about the binding conditions.}	290	2-13	TSKgel SP-3PW^{04TOSOH Biosep LLC (former TosoHaas)}
30	30 (20-40)	Methacrylic polymer^{B2Supplier reports that volume changes due to ionic strength or pH are negligible.}	5,000kDa (1,000Å)	60-120	20-40^{E1No data about the binding conditions.}	290	2-13	TSKgel SP-5PW (30µm)^{04TOSOH Biosep LLC (former TosoHaas)}
31	(20-40)	Methacrylic polymer, cross-linked, proprietary surface extender^{B1Supplier does not provide data about volume changes due to ionic strength or pH.}	(800Å)	No data	150^{E1No data about the binding conditions.}	116	1-13	Fractogel SO₃(S)^{06EMD Millipore (Merck Millipore)}
32	25 [20^{AExperimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245.}]	Methacrylic polymer^{B6Supplier reports <1% volume changes at pH 4-10 and <5% volume changes for 0.01-1.0 M NaCl.}	(725Å)	90-150	(>40)^E17Dynamic.	100	1-12	Macro-Prep 25S^08Bio-Rad

^A-Experimental value determined by Coulter Counting, in A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193–245.

^B1-Supplier does not provide data about volume changes due to ionic strength or pH. ^B2-Supplier reports that volume changes due to ionic strength or pH are negligible. ^B3-Supplier reports <5% volume changes at pH 4-10 and for 0.01-1.0 M NaCl. ^B4-Supplier reports <3% volume changes at pH 4-10 and <9% volume changes for 0.01-1.0 M NaCl. ^B5-Supplier reports that volume changes due to ionic strength or pH are absent. ^B6-Supplier reports <1% volume changes at pH 4-10 and <5% volume changes for 0.01-1.0 M NaCl.

^C1-Mean pore diameter ISEC-calculated in:P. DePhillips, A.M. Lenhoff , Journal of Chromatography A, 883, 2000,39-54. ^C2-By analogy with mean pore diameter for Q Sepharose XL stated in: Y. Yao, A.M. Lenhoff , Journal of Chromatography A, 1037, 2004,273-282. ^C3-Supplier states that the bead and pore sizes are similar between Capto S and Q Sepharose™ Fast Flow. ^C4-Determined by inverse size exclusion chromatography: E. X. Pérez Almodóvar, Y. Tao, G. Carta, Biotechnol Prog. 27, 2011, 1264-72.

^D1-Supplier provides ionic capacity data as 2.6-3.4 meq/dry gram. Number in the table obtained by applying the experimentally established column packing density of 0.15 dry g/ml: P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J.Chromatography. A,160, 1991, 207. ^D2-Supplier provides ionic capacity data as 1 meq/dry gram. Number in the table obtained by applying the experimentally established column packing density of 0.22 dry g/ml: P. Levison, C. Mumford, M. Streater, A. Brandt-Nielsen, N.D. Pathirana, S.E. Badger, J.Chromatography. A,160, 1991, 207.

^E-Lysozyme: pI ~11, MW = 14.5 kDa; Human IgG: pI~7.4-8.6, MW = 150 kDa; Bovine COHb: MW = 69 kDa; Ribonuclease: pI~9.6, MW = 14 kDA; Insulin: pI~5.3, MW = 6kDa. ^E1-No data about the binding conditions. ^E2-Dynamic at 10 % breakthrough: A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193-245. ^E3-Dynamic at 10 % breakthrough. ^E4-Flow rate of 75 cm/h in 0.1 M acetate bugger pH 5. Only 1.1 mg/ml of IgG binding catacity at the same conditions. ^E5-Lysozyme: Dynamic at 10 % Breakthrough: A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193-245. Human IgG: Flow rate of 75 cm/h in 0.1 M acetate bugger pH 5. ^E6-Dynamic at 10 % Breakthrough. Flow rate of 600 cm/h in 30 mM sodium phosphate buffer, pH 6.8. ^E7-Lysozyme: batch adsorption, sodium phosphate buffer pH 6.5:E. X. Pérez Almodóvar, Y. Tao, G. Carta, Biotechnol Prog. 27, 2011, 1264–72. Human IgG: dynamic at 10 % breakthrough, flow rate of 300 cm/h, 4.5 mg/ml polyclonal human IgG in 40 mM sodium acetate and 30 mM sodium chloride, pH 5.0. ^E8-Lysozyme: no data about the binding conditions. Human IgG: dynamic, 2 min residence time. ^E9-Lysozyme: batch adsorption, sodium phosphate buffer pH 6.5:E. X. Pérez Almodóvar, Y. Tao, G. Carta, Biotechnol Prog. 27, 2011, 1264–72. Human IgG: 10 % breakthrough, flow rate of 150 cm/h, 4.5 mg/ml polyclonal human IgG in 40 mM sodium acetate and 30 mM sodium chloride, pH 5.0. ^E10-Human IgG: dynamic at 10 % breakthrough, flow rate of 150 cm/h, 4.5 mg/ml polyclonal human IgG in 40 mM sodium acetate and 30 mM sodium chloride, pH 5.0. ^E11-Lysozyme: static. Human IgG: dynamic at 10 % breakthrough, flow rate of 212 cm/h, 1 mg/ml polyclonal human IgG in 100 mM acetate buffer. ^E12-2 Minute residence time, 5 mg/mL hIgG in 50 mM sodium acetate, pH 4.5. ^E13-Lysozyme: Dynamic at 10 % Breakthrough, A. Staby, J. Nielsen, J. Krarup et all, Advances in Resins for Ion-Exchange Chromatography, in Advances in Chromatography, Edited by Nelu Grinberg and Eli Grushka, Volume 47, 2009, 193-245. Human IgG: no data about the binding conditions. ^E14-Dynamic at 10 % breakthrough, flow rate of 200 cm/h, 5 mg/mL lysozyme in 50 mM sodium acetate, pH 4.5. ^E15-The supplier recommends the media for purifications of proteins in excess of 150kDa, and for purifications of PEGylated proteins containing at least 10kDa of PEG. ^E16-Dynamic at 10 % breakthrough, residence time of 4 min at flow rate of 250 cm/h, 20 mM sodium phosphate, pH 7.2. ^E17-Dynamic.

^F1-By analogy with data for Q-Cellthru Bigbeads Plus: A. Staby, I. H. Jensen, Journal of Chromatography A, 908, 2001, 149-161. ^F2-Value is provided in the Tosoh website. Material data sheet (downloaded from the same website) give max pressure value of 102 psi.

^G1-Working pH range.

⁰¹-Sterogene ⁰²-GE Life Sciences (former Amersham biosciences) ⁰³-Whatman (part of GE Healthcare) ⁰⁴-TOSOH Biosep LLC (former TosoHaas) ⁰⁵-JNC corporation, Japan ⁰⁶-EMD Millipore (Merck Millipore) ⁰⁷-PALL Life Sciences (former BioSepra) ⁰⁸-Bio-Rad

Protein Purification by Ion-Exchange Chromatography

List of Commercially Available Strong Cation Exchangers*

List of Commercially Available Strong Cation Exchangers^*